Western blotting is a powerful protein detection tool, but small mistakes can ruin results. From sample prep to antibody incubation, every step matters. Learning how to troubleshoot issues like high ...

Western blots can often be overlooked as a commonplace research tool in proteomics, but there have been continual updates and progress in the technical development of the western blot and the list of ...

Western blotting (or immunoblotting) is a well-established biochemical technique that detects specific proteins in complex samples and has applications from basic research to the diagnosis of ...

We understand that gaining publication-quality results straight away is not the norm when performing western blot. This 32-page handy guide provides tips and tricks to obtain high-quality, publishable ...

Proteins are the molecular machinery of a cell, ensuring that every cell in the body can carry out its specific role. Scientists often wish to study proteins and one way to achieve this is by western ...

Verify that the antibody is definitely able to detect the antigen in the species by checking data sheets and reference material Potential Cause: Primary and secondary antibody mismatch Raise the ...

A capillary western blot (Wes) technology has recently been validated for analyses of cell culture lysate proteins, but whether it is reliable for human tissue proteins is unknown. We compared ...

Immunohistochemistry and Western blotting both work by exploiting the principle of how antibodies specifically bind to the antigens present in biological tissue. Immunohistochemistry is the most ...

Add The Scientist as a preferred Google source to see more of our trusted coverage. In this webinar, Celeste Nelson and Oscar Abilez will discuss some of the latest approaches to overcoming setbacks ...

As protein analysis workflows become increasingly central to drug discovery, gene therapy development, and translational research, laboratories are under pressure to deliver more data, at higher ...

The BlotCycler is a simple and elegant system designed to perform blocking, addition, and incubation of primary and secondary antibodies, and washing steps automatically. It is equipped with trays ...